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pr plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pr plasmid
    Pr Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmids+42875/pCRISPR+(Plasmid+%2342875)/pm40999904-55-23-27
    Average 93 stars, based on 50 article reviews
    pr plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Amplification:

    Article Title: An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine
    Article Snippet: DNA constructs were sequenced by ACGT, Inc. Reagents and chemicals were obtained from Sigma-Aldrich and Fisher Scientific, unless otherwise noted. .. New England BioLabs supplied restriction enzymes and T4 DNA ligase. pCRISPR and pCas9 were obtained from Luciano Marraffini (Addgene plasmids 42875 and 42876). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Bacterial strains, plasmids, and oligonucleotides used in this study The fabG Δ 8 gene was amplified from strain SW01 via PCR using primers fabG BspHI F and fabG SphI R. The product was digested with the restriction enzymes BspHI and SphI and inserted into the NcoI and SphI sites of vector pBAD24 to generate pSW01. .. The wild-type fabG gene was amplified from E. coli MC1061 by using the same primers and vector to give pSW02.

    Polymerase Chain Reaction:

    Article Title: An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine
    Article Snippet: DNA constructs were sequenced by ACGT, Inc. Reagents and chemicals were obtained from Sigma-Aldrich and Fisher Scientific, unless otherwise noted. .. New England BioLabs supplied restriction enzymes and T4 DNA ligase. pCRISPR and pCas9 were obtained from Luciano Marraffini (Addgene plasmids 42875 and 42876). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Bacterial strains, plasmids, and oligonucleotides used in this study The fabG Δ 8 gene was amplified from strain SW01 via PCR using primers fabG BspHI F and fabG SphI R. The product was digested with the restriction enzymes BspHI and SphI and inserted into the NcoI and SphI sites of vector pBAD24 to generate pSW01. .. The wild-type fabG gene was amplified from E. coli MC1061 by using the same primers and vector to give pSW02.

    Plasmid Preparation:

    Article Title: An Eight-Residue Deletion in Escherichia coli FabG Causes Temperature-Sensitive Growth and Lipid Synthesis Plus Resistance to the Calmodulin Inhibitor Trifluoperazine
    Article Snippet: DNA constructs were sequenced by ACGT, Inc. Reagents and chemicals were obtained from Sigma-Aldrich and Fisher Scientific, unless otherwise noted. .. New England BioLabs supplied restriction enzymes and T4 DNA ligase. pCRISPR and pCas9 were obtained from Luciano Marraffini (Addgene plasmids 42875 and 42876). table ft1 table-wrap mode=article t1 TABLE 1 caption a4 Bacterial strains, plasmids, and oligonucleotides used in this study The fabG Δ 8 gene was amplified from strain SW01 via PCR using primers fabG BspHI F and fabG SphI R. The product was digested with the restriction enzymes BspHI and SphI and inserted into the NcoI and SphI sites of vector pBAD24 to generate pSW01. .. The wild-type fabG gene was amplified from E. coli MC1061 by using the same primers and vector to give pSW02.



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